This stage sees sides from the Highland League, Lowland League, East of Scotland League and South of Scotland League enter the competition along with the two preliminary-round qualifiers, Musselburgh Athletic and Lothian Thistle Hutchison Vale.
Here is our interactive map showing where you can see all the matches being played across the country.
There are some exciting encounters taking place too.
East Stirlingshire host Inverurie Locos in a mouth-watering tie as one of the the Lowland League's top sides goes head to head with a Highland League heavyweight.
Edinburgh’s Spartans take on East Kilbride in an all Lowland League tie, while there's a local derby in Aberdeenshire as Turriff United make the short trip to Formartine United.
Camelon have a chance at a cup upset as they head to Highland League side Deveronvale, while two overachievers last season, Broxburn Athletic and BSC Glasgow, are away to Newton Stewart and at home to Haddington Athletic respectively.