The schedule will follow the traditional format of a group stage consisting of five match days followed by four rounds thereafter culminating with the final at Hampden Park scheduled for Sunday 28 February, 2021.
The full dates are:
Matchday 1 – Tuesday 6 October, 2020
Matchday 2 – Saturday 10 October, 2020
Matchday 3 – Tuesday 13 October, 2020
Matchday 4 – Tuesday 10 November, 2020
Matchday 5 – Saturday 14 November, 2020
Saturday 28 and Sunday 29 November, 2020
Tuesday 15, Wednesday 16 and Thursday 17 December, 2020
Saturday 23 and Sunday 24 January, 2021
Sunday 28 February, 2021
A statement from the SPFL said: "Commencing on Tuesday October 6 with the Round 1 group stage, the competition will once again follow the traditional format over five rounds, concluding on 28 February 2021 with the final at Hampden Park.
"All 42 SPFL Clubs have now confirmed that they are willing and able to take part in this season’s Betfred Cup. We will shortly be liaising with the Highland League and Lowland League about the possibility of their champion clubs from last season, Brora Rangers and Kelty Hearts, participating as well.
"The group stage will follow the same format as last year, with matches resulting in a draw after 90 minutes going straight to a penalty shoot-out, with the winners earning an extra ‘bonus point’ in addition to the traditional point given for a draw. The eight group winners and four best runners-up will then progress to the last 16 knock-out round.
"The draw for the first round group stage fixtures will take place in due course, with further details to follow."
SPFL Chief Executive Neil Doncaster said: “Last season’s Betfred Cup was a hugely successful competition and we are looking forward to the same combination of excitement and passion this season.
“The process of scheduling the SPFL’s premier cup competition has been made much more difficult this year due to the disruption caused by Covid-19, but we are delighted to be in a position to confirm five uninterrupted rounds of fixtures once again.
“The innovations made to the competition in the last few seasons have proved to be a real winner with clubs and supporters alike.
“We are extremely grateful to our friends at Premier Sports, Betfred and the Scottish FA for their flexibility in agreeing this season’s Betfred Cup schedule.
“We wish all the clubs the best of luck in their quest to reach next year’s final.”