The incident took place on the M876 eastbound, prior to Junction 3 Bowtrees, around 5.20pm yesterday and involved a white Vauxhall Corsa which left the carriageway and continued down a steep embankment before stopping in some trees.
The vehicle had been heading towards the Kincardine Bridge.
Emergency services attended the scene and a 52-year-old woman was taken to Forth Valley Royal Hospital in Larbert with serious but non-life-threatening injuries, while the man, 45, was treated for minor injuries.
Inspector Peter Houston, of Forth Valley Road Policing Unit, said: “We are appealing to other road users who may have witnessed the crash to get in contact with police.
“I would ask if anyone was driving in the area at the time or has possible dashcam footage that could assist with our investigation that they get in touch with officers.”
Contact 101. Quote incident number 2340 of February 24.