Falkirk has voted against independence by a majority of over 7500.
And the majority of the country has followed suit with Deputy First Minister Nicola Sturgeon conceding defeat just after 5 a.m. with only a few results awaited.
The announcement of another No victory from Fife just after 6 a.m. confirmed the inevitable.
The local declaration was made by Counting Officer Rose Mary Glackin in Grangemouth Sports Complex at 4.19 a.m. to loud cheers from the No camp.
Yes took 50,489 votes (46.4 per cent) with 58,030 (46.4 per cent) opting for No.
There were 107 rejected papers, including 14 people who had voted for both Yes and No.
Turnout at the polls across the district was a mammoth 88.7 per cent with 108,626 votes cast.
Labour’s Craig Martin, Falkirk Council leader, said: “This turnout tells you the people of Falkirk were engaged in this Referendum and have listened to both sides.
“What they have done in Falkirk shows the expectations for the promises that were given to them.
“I am sure the Labour Party will be at the forefront of delivering on those promises.”
Angus MacDonald, the SNP’s Falkirk East MSP, said; “Clearly it’s a disappointing result given the work the work that has been done by activists of all parties and none.
“It’s certainly unfortunate there seems to have been unfair tactics and misinformation given, particularly at the tail end of the campaign, but I’m sure these issues will be addressed in the future.”