Work being carried out by Scottish Water, Scottish Gas, and Falkirk Council has closed part of the B816 Seabegs Road which connects Allandale and Bonnybridge.
A four-mile diversion route is in now in place, but Allandale businesses say losing passing trade is having a severe impact.
Margaret Craig, from Craig's Licenced Grocers, says she may be forced to close this weekend until the work is done.
She said: "My takings were down £1000 in just one day. I don't see how I can stay open."
Restaurant owner Johnny Sandhu from Underwood Lockhouse said: "I'm down 40 per cent already, and I think it's only going to get worse.
"This road is a main route for people going to the Falkirk Wheel, so the summer months are our busiest."
* A shuttle bus is now in operation in Allandale. To see the full timetable, visit
http://www2.falkirktoday.co.uk/Allandale&GreenhillShuttleJUN08.pdf
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